The combination of decitabine with multi-omics confirms the regulatory pattern of the correlation between DNA methylation of the CACNA1C gene and atrial fibrillation.

In summary, these findings suggest an increasing trend in the number of molecular players involved in the pathogenesis of AF as the disease progresses.

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Wilcoxon tests revealed significant differences in DNA methylation levels between the AF and SR groups at Site_1, Site_3, and Site_M (mean of the three sites) ( P < 0.05), while Site_2 showed non-significant but near-significant differences ( Figure 3G ).

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