Single-nucleus transcriptomic profiling of the diaphragm during mechanical ventilation.

Western blotting analysis revealed that the protein expression of PFKFB3, PDGFD, and CXCR2 tended to increase and that the protein expression of SEMA3A, NEGR1 and MEF2C tended to decrease in the experimental group compared with the control group, but these differences did not reach statistical significance (Fig. 8 ).

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