Patient-derived response estimates from zero-passage organoids of luminal breast cancer.

Among factors, patient, day of culture, and the patient × day interaction were highly significant, reflecting inter-tumor differences in growth and the increases in organoid size over two weeks (Fig. 4 A–C).

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Notably, volume effects did not reach statistical significance when 5 μl and 7 μl cultures were compared, indicating negligible differences between these droplet sizes (Fig. 4 D).

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