Furthermore, immunoblots of testicular lysates from 11-week-old rats using three different anti-BRCA2 antibodies, each of which recognizes regions upstream of mutation point, downstream of mutation point and phosphorylated BRCA2, respectively, showed a trend of decreased expression of BRCA2 in Brca2 wt/mut .
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Heterozygous mutation in BRCA2 induces accelerated age-dependent decline in sperm quality with male subfertility in rats.
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