An analysis of the nuclear fraction, which is defined as the fraction of cellular N-WASP (integrated cellular HA intensity) present in the nucleus, indicated a strong decrease in nuclear localization for the tyrosine phosphorylation mimicking the Y253D mutant compared to WT, while the non-phosphorylated Y253F mutant was distributed quite similarly to WT and only showed a trend of increased nuclear localization ( Figure S2B ).
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Nuclear N-WASP Induces Actin Polymerization in the Nucleus with Cortactin as an Essential Factor.
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