As with the preliminary signature from the broad-spectrum analysis, in our model, animals from the 20 Gy group were less distinguishable from the others although the AUC was close to significance ( p-value = 0.06).
However, at day 14 post-irradiation, despite a slight trend of increasing perfusion in irradiated limb, there was no significant difference between experimental groups (Fig. 1 g, h).
The performances of the 6-metabolite panel to discriminate the experimental groups from one another were highly significant to ensure the relevance of the signature for the diagnosis of LRI.
At day 14 post-irradiation (end of experiment), the observational injury score showed a trend of increasing severity with increasing dose (Fig. 1 c, d).