Reprogramming the melanoma and immunosuppressive myeloid cells with esomeprazole-loaded PLGA nanoparticles.

LysoTracker, which is largely independent of pH, stains the acidic compartments for size, number, and content of the acidic organelles, showed an increasing trend for ESO-NP-treated cells when compared to eNP-treated ones ( Figure 5 B).

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The LysoTracker levels displayed a decreasing trend during the observation period and reached the lowest levels at the 72-h time point ( Figure 3 C), which could be associated with the continuous nutrient consumption during cell proliferation.

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We observed that compared to eNPs, ESO-NPs induced a reduction of melanin, even though this effect did not reach statistical significance.

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