Efficient Dual Cas9 Nickase Correction of a Prevalent Pathogenic <i>LAMB</i> <i>3</i> Variant for Junctional Epidermolysis Bullosa.

On average, incorporation of the distant sgRNA1-blocking SNV increased from 20.6% to 42.6% of sequencing reads for Cas9-sgRNA2 editing when comparing the same 2 templates; however, this difference did not reach statistical significance ( P = .06) ( Figure 2 b and d).

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