Effect of Feeding Frequency on the Growth, Body Composition, and Intestinal Health of Hybrid Grouper (<i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatu</i>♂) Fed a High-Fat Diet.

The increase in feeding frequency led to a decreasing trend in crude ash, with the 1 time/day group being significantly higher than the other groups ( p < 0.05), and no significant differences between the 2 times/day, 3 times/day, and 4 times/day groups.

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Trypsin (TPS) showed a trend of first decreasing and then increasing, with the minimum value in the 2 times/day group, significantly lower than the other groups ( p < 0.05), and the maximum value in the 4 times/day group, significantly greater than the 1 time/day group ( p < 0.05), with no significant difference compared to the 3 times/day group ( p > 0.05), and no significant difference between the 1 time/day group and the 3 times/day group (p < 0.05). 3.4.

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As feeding frequency increases, crude protein tends to rise and then stabilize, while crude lipid shows an increasing trend, with the highest value at 4 times/day.

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