Germ cell quantification in human fetal and prepubertal testis tissues: a comparison of current methodologies.

Furthermore, when tissue sections were quantified using Methods 3 and 4, respectively, a similar trend toward a reduction in germ cells was observed, but this did not reach statistical significance for samples exposed to cisplatin (67.28 ± 13.29 vs 78.23 ± 9.29 germ cells per mm 2 , P = 0.3101; Fig. 2C ) (435.7 ± 267.7 vs 582.7 ± 179.8 germ cells per mm 2 , P = 0.3505; Fig. 2D ) or carboplatin (64.54 ± 13.40 vs 78.23 ± 9.287 germ cells per mm 2 , P = 0.1344; Fig. 2C ) (390.4 ± 166.7 vs 582.7 ± 179.8 germ cells per mm 2 , P = 0.390; Fig. 2D ), when compared with control.

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