Selection and validation of reference genes in alfalfa based on transcriptome sequence data.

Under alkaline stress conditions, when selecting the most suitable and stable candidate reference gene and genome, the expression level of phyA showed a trend of first decreasing and then increasing with the aggravation of alkaline stress, but there was no significant difference between the groups ( P > 0.05).

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Under high-temperature stress, phyA expression level using three different internal reference genes showed an increasing trend at H4 and H8, a decreasing trend at H12, and a further increasing trend at H16 with the duration of high temperature.

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