Leveraging Circular Polymerization and Extension Cloning (CPEC) Method for Construction of CRISPR Screening Libraries.

Bio Protoc · 2025 · PMC11865837 · PMID 40028016

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closest p

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quite significantno p-value reported

Solution 2: For 1 μg of PCR-linearized product, usage of 1 μL of Esp3I (BsmBI) is quite significant; do not reduce the enzyme amount.

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