Electrostatics of salt-dependent reentrant phase behaviors highlights diverse roles of ATP in biomolecular condensates.

Experimentally, only WT exhibits a clear trend toward reentrant dissolution of condensed droplets (with a LLPS propensity plateau at [NaCl] ≈ 1.55−2.5 M, Figure 3a , blue data points), whereas the LLPS of 4Rto4K M and 4Rto4K C commences at [NaCl] ≈ 1.3 M, LLPS propensity then increases with [NaCl] (a trend consistent with the MD-predicted increasing LLPS propensity at low [NaCl]s in Figure 7b and c ), but no sign of reentrant dissolution is seen up to the maximum [NaCl] = 2 M investigated experimentally for the RtoK variants (Figure 9B of Wong et al., 2020 ).

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