2 ) but remaining at the lower end of the overall copy numbers (0.05–0.2 copies/μl) and showing no significant pattern of eDNA signal accumulation over time except a decreasing trend for nylon, highly influenced by the high initial copy numbers ( p ≤ 0.0019, Fig. 3 ).
Similarly, while not significant, a negative trend was observed for the NIS ASVs except for the sponge water samples, which indicated some increase over time, also not significant ( Fig. 4B ).
6 , the progression of beta-diversity dissimilarity shows an increasing trend over time for all matrices (10–720 min), although for nylon it nearly reaches a plateau at 360 min.