Scale-Up of Human Amniotic Epithelial Cells Through Regulation of Epithelial-Mesenchymal Plasticity Under Defined Conditions.

However, the flow cytometry results for CD9 showed a consistently high positivity rate of ≈100% upon culture passage and did not show a decreasing trend, suggesting that the decline in CD9 expression did not exceed the threshold of the flow cytometry assay within the passages we tested.

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The violin plots of marker expression at different passages showed (Figure 4G ) that the expression of the epithelial marker CD9 gradually decreased with culture passages, and the expression of CD326 showed a trend that first increased until passage P0 and then decreased with further culture passages, which was consistent with the trend of proliferation‐related genes (Figure 2H ).

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