As explained in detail in the appendix, although a clear trend is observed, a closer inspection of the raw data points towards several possible problems with this methodology that may compromise a quantitative interpretation of the results, namely, a strong quenching of CBF fluorescence by rifampicin due to trivial inner filter effects and/or effects dependent on the proximity of rifampicin and CBF such as FRET due to the strong overlap between CBF fluorescence and rifampicin absorption spectra ( Figure A3 ).
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The sentences
In recent years, there has been a notable trend toward increasing the molecular weight of drug candidates to improve specificity for their molecular targets [ 2 , 3 ].