showed a trendp < 0.05
ruthenicum roughage addition to the diet ( Figure 3B ); the relative abundance of the NK4A214_group showed a trend of increasing and then decreasing ( p < 0.05) with increasing levels of L. ruthenicum branches additions in the diet ( Figure 3C ); and the relative abundance of sheep ruminal microbiota phylum level species among groups had no significant effect ( p > 0.05) ( Figure 3A ).