New Insights into the Mediation of Biofilm Formation by Three Core Extracellular Polysaccharide Biosynthesis Pathways in <i>Pseudomonas aeruginosa</i>.

A test level of p < 0.05 was considered significant, and p < 0.01 was considered highly significant. 5.

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Conversely, the expression of DGCs follows varied trajectories: the upregulation of siaD contributes to increased c-di-GMP, whereas wspR shows a decreasing trend in expression.

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Following a one-hour inoculation with bacterial solutions and subsequent continuous perfusion with LB medium, strain PAO1-WT exhibited a negative trend in the frequency shift parameter (f3), which decreased over time.

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