However, there was no significant difference in non-progressive forward motility across the three methods ( Figure 2C ), indicating that microfluidic sorting primarily enhances the progressive movement of sperm, which is more relevant for fertilization success ( Figure 2D ). In terms of sperm morphology, microfluidic sorting resulted in a higher percentage of normal sperm forms compared to density-gradient centrifugation, although this difference did not reach statistical significance.
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Comparative Efficacy of Swim-Up, Density-Gradient Centrifugation, and Microfluidic Sorting in Sperm Preparation, and the Impact on Motility, Morphology, and DNA Integrity.
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