Soil depth determines the microbial communities in <i>Sorghum bicolor</i> fields within a uniform regional environment.

As soil depth increased to 30–60 cm, 60–90 cm, and beyond, the influence of field conditions on microbial community structure remained significant but showed a decreasing trend.

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bicolor fields Spearman’s rank correlation analyses revealed a significant negative correlation between soil depth and each alpha diversity metric (Faith’s phylogenetic diversity, Shannon’s entropy, and the observed features), indicating a strong trend of decreasing microbial diversity with depth.

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