Exogenous Gibberellins and Auxins Promote Crown Bud Regeneration and Influence Endogenous Hormone Changes in Alfalfa.

For both varieties, the endogenous ABA content showed a decreasing trend over time following the application of exogenous GA 3 and IAA, reaching the highest level after 5 days, significantly higher than at other times ( p < 0.05).For both ‘Xinmu No. 4’ and ‘Saiwo’, under the treatment with exogenous growth regulators GA 3 and IAA, the endogenous ABA content in the crown buds decreased with increasing concentrations.

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For both varieties, the endogenous IAA content showed an increasing trend over time following the application of exogenous GA 3 and IAA, reaching the highest level after 15 days, significantly higher than at other times ( p < 0.05).

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For “Saiwo”, under both GA 3 and IAA treatments, the endogenous GA 3 content showed a trend of initially increasing and then decreasing, with the highest level during the third observation period under G30 for GA 3 treatment, and the highest level across all periods under I15 for IAA treatment, significantly higher than CK ( p < 0.05). 2.2.2.

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