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PAM-free hairpin target binding activates trans-cleavage activity of Cas12a.

Nucleic Acids Res · 2025 · PMC12188295 · PMID 40557869

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showed a trendno p-value reported
4H ), but the distal loop showed a trend of initially increasing and then decreasing after exceeding a loop size of 8 nt (Fig. 4J ).

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