highly significantP < 0.01
Slaughter batches had a highly significant impact on the DL phenotype ( P < 0.01).
Slaughter batches had a highly significant impact on the DL phenotype ( P < 0.01).
Gender showed no significant effect on DL ( P = 0.06), though there was a trend towards significance.
KEGG pathway enrichment analysis revealed that PPARGC1A and TBC1D1 genes were enriched in the AMPK signaling pathway, a crucial pathway in muscle oxidative stress, showing a trend toward significance with a corrected P -value of 0.063 ( Fig. 3 and Table 5 ).
Meanwhile, for a few trait-related pathways, when the P -value reached a significant trend, this study was also reserved for subsequent analysis.