Ions with Ions, Entities with Entities: A Proof-of-Concept Study Using the SELM-1 Yeast Certified Reference Material for Intra- and Extracellular Se Quantification via Single-Cell ICP-Mass Spectrometry.

As can be seen, the NP-based calibration strategies for intracellular Se content determination are not only more direct and user-friendly (no measurement of ionic standards is required), but the results are more repeatable between working sessions and accurate as compared to the bulk analysis and certified value (R% of 102 ± 2% and 101 ± 3% for the external calibration and relative method, respectively). There also seems to be a clear trend for the particle size method relying on the use of AuNPs for the determination of the TE to overestimate the intracellular, and subsequently, the total Se content (R% = 108 ± 6%, ranging from 101% to 118%).

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