CRISPRa-Mediated Increase of OPA1 Expression in Dominant Optic Atrophy.

Additional analyses were also performed on the predicted CRISPRa activity with different factors, such as gRNA length, with a slight trend showing increased activity, with longer gRNAs going up to 23 bp [ 39 ].

also in 4,078 other papers

The addition of emetine increased the levels of OPA1 transcripts compared to the untreated samples; however, this change did not reach statistical significance ( Supplementary Table S1 ), indicating the likely involvement of NMD in the degradation of the mutant transcript despite the limited sample size and treatment time.

also in 111,027 other papers