Integrated physiological, biochemical and transcriptomic analyses elucidate the response of alpine plant Lamiophlomis rotata (Benth.) Kudo to Low-Nitrogen stress.

ANOVA and Duncan’s test were conducted for variance analysis ( P < 0.05 was considered significant and P < 0.01 was considered highly significant), and graphs were generated using GraphPad Prism 7.0.

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At 20 d of stress, root vitality showed an increasing trend, but it remained lower than that at 0 d of stress.

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By day 30, with the increase in stress intensity, CAT activity showed a trend of sharply increasing followed by inhibition: compared to the CK, CAT activity in the N1, N2, N3, and N4 groups increased by 93.30%, 498.04% (peak), 259.22%, and 4.75%, respectively.

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Compared to CK, the total nitrogen content in plants generally showed a decreasing trend with the increase in stress intensity on days 10, 20, and 30.

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