Optimization of Fermentation Conditions for the Production of 2,3,5-Trimethylpyrazine by Recombinant <i>Bacillus licheniformis</i>.

In the regression model, the primary items B and C were significant with p < 0.05; while, the primary item A, the interaction terms AC and BC, and the secondary terms A2, B2 and C2 were highly significant with p < 0.01, demonstrating that these factors and their interaction had an important influence on the TMP yield.

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The single-factor experiments found that with the change in the ratio of D-glucose to L-threonine, the yield of TMP showed a trend of increasing first and then decreasing ( Figure 5 A).

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