Integration of co-culture conditions and 3D gelatin methacryloyl hydrogels to improve human-induced pluripotent stem cells-derived cardiomyocytes maturation.

Front Bioeng Biotechnol · 2025 · PMC12301886 · PMID 40727646

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Cells co-cultured in the 3D system showed a trend of reduced oxidative stress in association with a significantly lower release of LDH enzyme and cTnI, indicating a less critical condition for cell survival and function in the 3D system with HCAECs with respect to the 2D hiPSC-CMs monoculture.

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