approaching significancep = 0.058
None of the treatments affected ENS frequency in triploids in the two other experiments (Tables 2 and 3 ), although acclimation temperature was approaching significance in Experiment 2 ( p = 0.058). 4 DISCUSSION Our study investigated whether rearing conditions, i.e. acclimation temperature or oxygen level, followed by acute aerobic stress, affect ENS frequency in diploid and triploid fish using brook charr as the experimental model.