Integrative identification of key genes governing <i>Verticillium</i> wilt resistance in <i>Gossypium hirsutum</i> using machine learning and WGCNA.

Clusters C3 (5,113 genes) and C5 (7,023 genes) showed a trend of sustained or delayed upregulation, reaching their highest expression at 48 h.

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Correlation analysis revealed that the blue module, turquoise module, and yellow module had module eigengenes strongly positively correlated with time (Pearson correlation coefficients 0.60, 0.57, and 0.24, respectively; p values all highly significant, Figure 3A ), meaning the genes in these modules showed continuously increasing expression as infection time progressed.

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