Evaluation of the viability and functionality of human peripheral blood mononuclear cells cryopreserved up to 2 years in animal-protein-free freezing media compared to the FBS-supplemented reference medium.

Front Immunol · 2025 · PMC12355051 · PMID 40821813

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did not reach statistical significanceno p-value reported

In contrast, a trend toward decreased cytokine release was observed in DMSO-free media, though differences did not reach statistical significance, likely due to substantial inter-donor variability.

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