Genetic Drivers of Plant Root Colonisation by the Biocontrol Agent Pseudomonas protegens Pf-5.

A large number of the genes (18 out of 30) for which loss of function significantly reduced root colonisation fitness for wheat also showed a trend for reduced cotton root colonisation but fell below the cutoffs used in this work exhibiting either a log 2 ‐FC < −2, but a q ‐value > 0.01 (although these had q ‐values < 0.05, or a log 2 ‐FC less than −1.9, but still greater than −2) (Figure 1 , Table S4 ).

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The Pf‐5 gene PFL_0517, encoding a serine/threonine protein kinase, was highly significant for both plant colonisation assays (Figure 2 ).

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