Besides LDH changes, some analytes, such as calcium and cholesterol, showed near-significant changes in elder participants, and their samples appeared to be more sensitive to a delay in measurement and storage temperature.
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Biochemical analytes in centrifuged blood samples could be affected by the age of subjects in different time periods and storage temperatures.
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showed that calcium levels rise at 4°C and 8°C, which is in agreement to the results by the current report.[ 15 ] Besides, the results of a meta-analysis have showed that LDH is unstable at RT for 12 h with an increasing trend.