Freezing of Vaginal Swabs Prior to DNA Purification Does Not Statistically Significantly Affect Microbiome Composition.

Microbiologyopen · 2025 · PMC12394732 · PMID 40878290

Europe PMC DOI

hedged sentences

0.0610

closest p · 1.2× alpha

0.0610

boldest claim

The sentences

a trend toward significancep = 0.061so close (0.05 < p ≤ 0.1)

In contrast, a separate beta diversity (Figure 4 ) analysis using an ANOVA‐based approximation of permutational multivariate analysis of variance (PERMANOVA) on Bray–Curtis distances revealed a trend toward significance ( F = 3.51, p = 0.061), suggesting possible group‐level differences in microbial community structure (Figure 4 ).

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showed a trendp = 0.061so close (0.05 < p ≤ 0.1)

PERMANOVA showed a trend ( F = 3.51, p = 0.061), but ANCOM‐BC2 found no differentially abundant taxa.

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