Coverage analysis revealed that Settings 2 and 3 were able to use most of the demultiplexed reads to call the haplotype (i.e., trend close to a slope of one), while Setting 1 showed a trend with a slope close to 0.5 instead of close to one (Figure 4A–C ).
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CCS-Consensuser: A Haplotype-Aware Consensus Generator for PacBio Amplicon Sequences.
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