Development and Application of a Novel pH-Responsive Bilayer Indicator Film for Yellowfin Seabream Preservation and Freshness Monitoring.

After regression analysis was performed, the quadratic polynomial regression equation describing the effects of CAR/GEL mass ratio (A), CS concentration (B), and GSA/CUR dosage (C) on the comprehensive score (Y) was obtained as follows: (11) Y = 0.66 − 0.041A + 0.019B + 0.036C + 0.061AB + 0.075AC + 0.027BC − 0.054A 2 − 0.091B 2 − 0.054C 2 Analysis of variance (ANOVA) for the response surface methodology (RSM) experiments ( Table 7 ) revealed the following: The regression model was highly significant ( p = 0.0002).

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Factor B (CS concentration) alone was not significant ( p = 0.0575), but its interactions with A or C (AB, BC) approached significance ( p -values close to 0.05).

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