A negative trend was observed in oxidative stress indicators, while sperm viability exhibited a slight positive trend, suggesting that oxidative stress may have a greater impact on older frozen samples.
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Effects of long-term cryopreservation on sperm quality and oxidative stress in Manchega rams (Ovis aries).
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The sentences
A positive trend was observed in the percentage of viable spermatozoa relative to generation, while a negative trend emerged in spermatozoa entering early apoptosis relative to generation.