Spatial mapping of cells both positive for Ki67 and Pan-cytokeratin revealed a clear trend: the number of actively proliferating cancer cells was lowest within stromal regions and progressively increased with distance from the stroma-tumor interface, plateauing at approximately 300 μm ( Figure 5D , Supplementary Figure S5B ).
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An image analysis pipeline to quantify the spatial distribution of cell markers in stroma-rich tumors.
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