Compared to 96-well plates, we quantified a drop from 80 to 45% of cells expressing T and 60–30% in Foxa2 in droplets, while Pax6 expression showed a trend of being higher in droplets, from 3.5% vs. 1%.
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Culture of pluripotent stem cells in microscale droplets modulates differentiation and tissue patterning towards organoids on chip.
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