Microbial response mechanisms of organic nitrogen substitution for optimizing tobacco yield and quality: the key role of 50% organic nitrogen.

The bacterial, fungal, and total microbial residue carbon all showed a decreasing trend of 60%ON > 50%ON > 40%ON > CK with the reduction of organic nitrogen content: at the HT site, all three organic nitrogen treatments were significantly higher than the CK; at the LY site, there were significant differences among the 60%ON, 50%ON, and 40%ON treatments, but no significant difference was found between the 40%ON treatment and CK ( Supplementary Figure S1 ). 3.4 Soil microbial community diversity and structure The application of organic nitrogen increased soil microbial diversity at both experimental sites.

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The lignin content at both sites showed a trend of 50%ON > 60%ON > 40%ON > CK, but there were no significant differences among the treatments ( Table 2 ).

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