Electroacupuncture ameliorates Autism Spectrum Disorder via modulating the gut-brain axis depending on the integrity of vagus nerve.

4C , P < 0.05), accompanied by a downward trend in IL-6 and IL-17A levels, though these changes did not reach statistical significance (Fig. 4A, B , both P > 0.05).

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In the open-field test, EA + FMT(ASD) mice demonstrated a significantly reduced total distance traveled in the central area compared to EA mice, while activity time showed a decreasing trend (Fig. 8C, D , total distance P < 0.05, activity time P > 0.05).

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Additionally, the number of interactions in this phase demonstrated an increasing trend.

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