Functional Validation of <i>ALDOA</i> in Regulating Muscle Cell Fate: Based on In Vitro Proliferation, Apoptosis, and Differentiation Experiments.

In porcine skeletal muscle satellite cells, the most pronounced effect was observed at a vector concentration of 5 μg, with the overexpression group showing a highly significant increase compared to the empty vector group ( p < 0.001) ( Figure 2 D).

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For cell proliferation-related markers, quantitative analysis revealed that although CDK2 and CDK4 expression levels in the overexpression group were lower than those in the control group, the differences did not reach statistical significance ( p > 0.05).

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After collecting cells at different time points and analyzing quantitative results, it was found that the ALDOA gene expression showed an increasing trend with the duration of differentiation.

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