Lipid Synthesis and Transcriptional Control ( Figure 9 b) For lipid metabolism-related transcription factors, Srebf1 showed a highly significant phase effect (F(1, 25) = 24.42, p < 0.0001, partial η 2 = 0.49, q = 0.0001), with higher expression at ZT3 than at ZT15, and a trend toward an exercise effect (F(1, 25) = 3.72, p = 0.0653, partial η 2 = 0.13) that was not confirmed after FDR adjustment.
Pnpla2 showed a trend toward a phase × exercise interaction (F(1, 25) = 3.67, p = 0.0672, partial η 2 = 0.13), with post hoc analysis indicating significantly lower expression in ZT3-exe than in ZT15-exe (mean difference = −0.67, t(25) = 2.78, 95% CI [−1.17, −0.17], p = 0.0105, Hedges’ g = 1.08), and lower levels in ZT15-sed than in ZT15-exe mice (mean difference = −0.51, t(25) = 2.03, 95% CI [−1.03, −0.01], p = 0.0521, Hedges’ g = 0.79); however, this interaction did not remain significant after FDR correction.
In contrast, Per2 showed only a weak trend (F(1, 25) = 2.98, p = 0.0967, partial η 2 = 0.11), which was not supported after FDR correction.