Key Nutrient Drivers for Biomass and C-Phycocyanin Production in <i>Spirulina</i> sp. Revealed by Media Optimization.

Although differences were small and statistically insignificant during the early phase (Day 3–6, p > 0.05), the effect became significant on Day 10 ( p = 2.93 × 10 −2 ) and highly significant on Days 13 ( p = 3.25 × 10 −3 ) and 14 ( p = 1.69 × 10 −5 ).

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On the other hand, increasing the phosphorus concentration from 0.25 g/L to 1 g/L showed a negative trend across PCL, PCD, and PI, as indicated by the downward-sloping curve in the main effect plot.

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