Role of SQSTM1/p62 in regulating Mallory-Denk body in alcohol-associated liver disease.

Western blot analysis indicated that the hepatic levels of α-smooth muscle actin (α-SMA) and CK19 showed an elevated trend in WT and p62 KO mice on the DDC diet ( figure 4D ).

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Consistent with the IHC staining data, western blot analysis showed increased levels of p62 and showed an increasing trend of levels of ubiquitinated proteins and CK8/CK18 in both detergent soluble and insoluble fractions in AH livers compared with healthy livers.

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Serum levels of ALT and AST but not ALP increased in alcohol-fed WT and p62 KO mice compared with control diet-fed mice, although the changes did not reach statistical significance.

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