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Systematic evaluation of CrRNA design parameters for optimized Cas13d-mediated RNA targeting in chicken cells.

Funct Integr Genomics · 2025 · PMC12647337 · PMID 41291135

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showed a trendno p-value reported
The truncated 24-nt to 21-nt crRNAs showed a trend of decreasing targeting efficiency, with a 2–3% loss observed for every nucleotide truncation.

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