Improving Gene Knock-In Efficiencies in Sheep Primary Cells Using a CRISPR/Cas9-Gal4 System.

While the knock-in efficiency of mCherry gene did not reach statistical significance ( p = 0.13), a notable enhancement was evident, with efficiency increasing by 2.43-fold from 5.10% (5/98) to 12.37% (12/97) ( Figure 3 B,D; Table S3 ).

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