3D printed scaffolds regulated by neural-bone metabolic coupling promote bone unit regeneration.

Meanwhile, we found that ester synthesis-related proteins associated with myelin synthesis such as Acat2/3 (Acetyl-CoA Acetyltransferase 2/3), Pmvk (Phosphomevalonate Kinase), Mvk (Mevalonate Kinase), Pcyox1 (Pyridoxal (Pyridoxine) 5′-phosphate Oxidase 1), Idi1 (Isopentenyl-diphosphate Delta-isomerase 1), Cspg4 (Chondroitin Sulfate Proteoglycan 4), Aspm (Abnormal Spindle Microtubule Assembly), Fdps (Farnesyl Diphosphate Synthase), and Plpp2 (Phospholipase B1 (PLB1) Phospholipase A2) also showed a trend of high expression ( Fig. 4 D).

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The results indicated that after co-culture with DRG, the expression of neurofactor receptors including Calcrl, Vipr, and Ghr was significantly up-regulated in BMSCs, accompanied by an increasing trend in the expression of downstream signaling molecules Erk1/2 and BMP-2.

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