Pre-Experimental Wet Heat Sterilization Alters the Ecotoxicity of Pristine Graphene Oxide Toward <i>Daphnia magna</i>.

Based on inhibition percentage data, although an increasing trend in the toxicity of untreated, autoclaved, and tyndallized GO samples could be observed, the calculated inhibitions relative to the control group were not statistically significant.

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The hydrodynamic diameter distribution results ( Figure S4 ) of the Daphnia growth medium, the GO stuck suspensions in distilled water (DW), and samples from the assembled test systems with GM suggest a large fraction of particles in the range of 1–2.5 μm, in many cases with a multi-peaked (highly heterodisperse) size distribution, so a clear trend could not be observed.

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