Single-cell transcriptomics reveals immune remodeling of the murine lung microenvironment following chronic house dust mite exposure.

Although some comparisons in the ELISA analysis did not reach statistical significance, the overall patterns mirrored those observed at the transcript level, supporting concordant regulation at both mRNA and protein levels.

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Tnf expression showed a decreasing trend in both qPCR and scRNA-seq analyses, consistent with the reduction in activated neutrophils and IFN neutrophils expressing Tnf ( Figures 4A–C , Supplementary Figure 5 ).

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